Two new terpenoids from the leaves of <i>callicarpa macrophylla</i>

Abstract

Two new terpenoids (<b>1</b>-<b>2</b>) and seven known compounds (<b>3</b>-<b>9</b>) were isolated from methanol extract of <i>Callicarpa macrophylla</i> leaves. Their structures were determined to be <i>ent</i>-7<i>α</i>,16<i>β</i>,17,18-tetrahydroxykaur-15-one (<b>1</b>), 3<i>β</i>-acetoxy-urs-12-ene-11-one-12-ol (<b>2</b>), <i>ent</i>-1<i>β</i>-acetoxy-7<i>α</i>,14<i>β</i>-dihydroxykaur-16-en-15-one (<b>3</b>), 3<i>β</i>-acetoxy-11<i>α</i>,13<i>β</i>-dihydroxyolean-12-one (<b>4</b>), <i>β</i>-amyrin (<b>5</b>), spinasterol (<b>6</b>), ursolic acid (<b>7</b>), <i>β</i>-sitosterol (<b>8</b>), and daucosterol (<b>9</b>) by analyses of their MS, NMR spectroscopic data and by comparison with those reported in the literature. Compounds <b>1</b> - <b>4</b>, and <b>7</b> displayed potential cytotoxic activity towards HepG-2, LU-1, and MCF-7 human cancer cell lines with IC₅₀ values ranging from 0.46 ± 0.21 to 18.14 ± 0.33 μM. Compound <b>6</b> showed IC₅₀ values of 14.17 ± 0.21 and 5.72 ± 0.42 μM against Hep-G2 and MCF-7 cell lines, respectively.