Enhanced Osteogenesis by Molybdenum Disulfide Nanosheet Reinforced Hydroxyapatite Nanocomposite Scaffolds

Abstract

The advances in the arena of biomedical engineering enable us to fabricate novel biomaterials that provide a suitable platform for rapid bone regeneration. Herein, we have investigated the in vitro and in vivo osteogenic differentiation, proliferation, and bone regeneration capability of molybdenum disulfide nanosheets (MoS2NSs) reinforced HAP nanocomposite scaffolds. The MG-63 cells were incubated with HAP and HAP/MoS2NSs nanocomposite and followed for various cellular activities. The cells incubated with HAP@2 shows higher cell adhesion, cell proliferation, and alkaline phosphatase activity (ALP) in contrast to HAP. The in vivo and in vitro results of the increased ALP level confirm that HAP@2 promotes osteogenic differentiation. This improved osteogenesis was validated with upregulation of osteogenic marker viz. transcription factor, RUNX-2 (∼34 fold), collagen-1 (∼15 fold), osteopontin (∼11 fold), osteocalcin (∼20 fold), and bone morphogenetic protein-2 (∼12 fold) after 12 week postimplantation in comparison to drilled. The X-ray imaging demonstrates that HAP@2 implants promote rapid osteogenesis and bioresorbability than HAP and drilled. The outcomes of the present study provide a promising tool for the regeneration of bone deformities, without using any external growth factor.