Alkaloids from the root of Indonesian <i>Annona muricata</i> L

Abstract

<i>Annona muricata</i> L. has been used traditionally in Indonesia to treat disease. Phytochemical studies on the alkaloid fractions from the root of <i>Annona muricata</i> L. from Malang-Indonesia resulted in the isolation of an unreported benzylisoquinoline alkaloid (+)-xylopine <b>5</b> as well as four known alkaloids (<b>1</b>-<b>4</b>). The crude methanol extract and alkaloid fractions were tested against <i>Plasmodium falciparum</i> K1 and against bacteria (<i>Escherichia coli</i>, <i>Klebsiella pneumonia</i>, <i>Acinetobacter buamanii</i>, <i>Pseudomonas aeruginosa</i>, Methicillin-resistant <i>Staphylococcus aureus</i>) with insignificant activities (MIC > 32 µg/mL). Individual alkaloids were tested against a human suspension cancer cell line (HL-60 leukemia cells) and two human fibroblastic cancer cell lines (A549 lung cancer cells and HepG2 liver cancer cells) in which compound <b>5 w</b>as the most toxic alkaloid with IC₅₀ values ranging from 20 to 80 µM.