RNAi as a Tool to Study Virulence in the Pathogenic Yeast Candida glabrata

Abstract

The yeast <i>Candida glabrata</i> is a major opportunistic pathogen causing mucosal and systemic infections in humans. Systemic infections caused by this yeast have high mortality rates and are difficult to treat due to this yeast's intrinsic and frequently adapting antifungal resistance. To understand and treat <i>C. glabrata</i> infections, it is essential to investigate the molecular basis of <i>C. glabrata</i> virulence and resistance. We established an RNA interference (RNAi) system in <i>C. glabrata</i> by expressing the Dicer and Argonaute genes from <i>Saccharomyces castellii</i> (a budding yeast with natural RNAi). Our experiments with reporter genes and putative virulence genes showed that the introduction of RNAi resulted in 30 and 70% gene-knockdown for the construct-types antisense and hairpin, respectively. The resulting <i>C. glabrata</i> RNAi strain was used for the screening of a gene library for new virulence-related genes. Phenotypic profiling with a high-resolution quantification of growth identified genes involved in the maintenance of cell integrity, antifungal drugs, and ROS resistance. The genes identified by this approach are promising targets for the treatment of <i>C. glabrata</i> infections.