Low Overnight Temperature-Induced Gibberellin Accumulation Increases Locule Number in Tomato

Abstract

The number of locules in tomato affects fruit size, shape, and the incidence of malformation. Low temperature increases locule number and the incidences of malformation in tomato plants. In this study, three flower bud developmental stages (pre-flower bud differentiation, sepal and petal primordium formation, and carpel primordium formation) under different night temperatures (10, 15, and 20 °C) were used to analyze the reason behind locule number change using an RNA sequencing (RNA-seq) approach, Quantitative real-time PCR (qRT-PCR), and ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS). The results showed that the "plant hormone signal transduction", "starch and sucrose metabolism", and "diterpenoid biosynthesis" categories were remarkably activated during flower bud differentiation. Transcripts of gibberellin (GA)-related genes and endogenous levels of GAs were analyzed, and it was discovered that <i>SlGA2ox</i> genes were significantly downregulated and bioactive GA₁ and GA₄ accumulated at lower overnight temperature. Exogenous application of bioactive GA₁, GA₄, and PAC (paclobutrazol) showed that GA₁ and GA₄ increased the locule number, while PAC decreased the locule number. Taken together, our results suggest that lower overnight temperature reduced the expression of <i>SlGA2ox</i> genes, leading to GA₁ and GA₄ accumulation, thereby increasing locule number in tomato.