A High‐Sensitivity 10‐Color Flow Cytometric Minimal Residual Disease Assay in B‐Lymphoblastic Leukemia/Lymphoma Can Easily Achieve the Sensitivity of 2‐in‐10⁶and Is Superior to Standard Minimal Residual Disease Assay: A Study of 622 Patients

Abstract

We report an easily reproducible high-sensitivity 10-color FC-MRD assay with the sensitivity of 2-in-10⁶ (0.0002%). It allowed the detection of low-level MRD in samples, which could have been reported negative using the standard FC-MRD with limited event analysis. Thus, this high-sensitivity MRD-methodology can provide a reliable basis for therapeutic modifications in B-ALL. © 2019 International Clinical Cytometry Society.