The Synthesis of RNA Containing the Modified Nucleotides<i>N</i>²-Methylguanosine and<i>N</i>⁶,<i>N</i>⁶-Dimethyladenosine

Abstract

Abstract The phosphoramidites of the naturally occurring modified nucleotides N 2-methylguanosine and N 6,N 6-dimethyladenosine were synthesized and incorporated into short oligoribonucleotides. Described are the syntheses of the phosphoramidites and the procedures used to deprotect oligoribonucleotides in which the O 6 of m2G is protected with a 2-(p-nitrophenyl)ethyl group.