Rotary substates of mitochondrial ATP synthase reveal the basis of flexible F ₁ -F _o coupling

Abstract

F₁F_o-adenosine triphosphate (ATP) synthases make the energy of the proton-motive force available for energy-consuming processes in the cell. We determined the single-particle cryo-electron microscopy structure of active dimeric ATP synthase from mitochondria of <i>Polytomella</i> sp. at a resolution of 2.7 to 2.8 angstroms. Separation of 13 well-defined rotary substates by three-dimensional classification provides a detailed picture of the molecular motions that accompany <i>c</i>-ring rotation and result in ATP synthesis. Crucially, the F₁ head rotates along with the central stalk and <i>c</i>-ring rotor for the first ~30° of each 120° primary rotary step to facilitate flexible coupling of the stoichiometrically mismatched F₁ and F_o subcomplexes. Flexibility is mediated primarily by the interdomain hinge of the conserved OSCP subunit. A conserved metal ion in the proton access channel may synchronize <i>c</i>-ring protonation with rotation.