Biosynthesis and characterization of magnesium oxide and manganese dioxide nanoparticles using <i>Matricaria chamomilla</i> L. extract and its inhibitory effect on <i>Acidovorax oryzae</i> strain RS-2

Abstract

Bacterial brown stripe (BBS) is one of the most economically important diseases of rice caused by <i>Acidovorax oryzae</i> (Ao). In order to ensure food security and safe consumption, the use of non-chemical approach is necessary. In this study, MgO and MnO₂ were synthesized using chamomile flower extract. The synthesized MgO and MnO₂ nanoparticles were characterized by UV-Visible spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction, transmission/scanning electron microscopy. The sizes were 18.2 and 16.5 nm for MgO and MnO₂ nanoparticles, respectively. The MgO and MnO₂ nanoparticles reduced the growth of Ao strain RS-2 by 62.9 and 71.3%, respectively. Also, the biofilm formation and swimming motility were significantly reduced compared to the control. The antibacterial mechanisms of MgO and MnO₂ nanoparticles against RS-2 reveals that MgO and MnO₂ nanoparticles penetrated the cells and destroyed the cell membrane leading to leakage of cytoplasmic content. Also, the flow cytometry observation reveals that the apoptotic cell ratio of RS-2 increased from 0.97% to 99.52 and 99.94% when treated with MgO and MnO₂ nanoparticles, respectively. Altogether, the results suggest that the synthesized MgO and MnO₂ nanoparticles could serve as an alternative approach method for the management of BBS.