Publication | Open Access
High-level extracellular production of recombinant nattokinase in Bacillus subtilis WB800 by multiple tandem promoters
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Citations
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References
2019
Year
Our studies demonstrated that NK was efficiently overproduced by tandem promoters in Bacillus subtilis. The highest fibrinolytic activity was promoted by P<sub>HpaII</sub>-P<sub>HpaII</sub>-P<sub>P43</sub>, which was much higher than that had been reported in previous studies. These multiple tandem promoters were used successfully to control NK expression and might be useful for improving the expression level of the other genes.
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