Strengths and limitations of size exclusion chromatography for investigating single chain folding – current status and future perspectives

Abstract

Synthetic approaches for Single-Chain Nanoparticles (SCNPs) developed rapidly during the last decade, opening a multitude of avenues for the design of functional macromolecular chains able to collapse into defined nanoparticles. However, the analytical evaluation of the SCNP formation process still requires critical improvements.