Dynamic and Functional Profiling of Xylan-Degrading Enzymes in <i>Aspergillus</i> Secretomes Using Activity-Based Probes

Abstract

, by the use of chemical probes inspired by the β-glucosidase inhibitor cyclophellitol. Furthermore, we demonstrate the use of these activity-based probes (ABPs) to assess enzyme-substrate specificities, thermal stabilities, and other biotechnologically relevant parameters. Our experiments highlight the utility of ABPs as promising tools for the discovery of relevant enzymes useful for biomass breakdown.