Na_V1.6 regulates excitability of mechanosensitive sensory neurons

Abstract

Peripheral sensory neurons express multiple voltage-gated sodium channels (Na_V ) critical for the initiation and propagation of action potentials and transmission of sensory input. Three pore-forming sodium channel isoforms are primarily expressed in the peripheral nervous system (PNS): Na_V 1.7, Na_V 1.8 and Na_V 1.9. These sodium channels have been implicated in painful and painless channelopathies and there has been intense interest in them as potential therapeutic targets in human pain. Emerging evidence suggests Na_V 1.6 channels are an important isoform in pain sensing. This study aimed to assess, using pharmacological approaches, the function of Na_V 1.6 channels in peripheral sensory neurons. The potent and Na_V 1.6 selective β-scorpion toxin Cn2 was used to assess the effect of Na_V 1.6 channel activation in the PNS. The multidisciplinary approach included Ca²⁺ imaging, whole-cell patch-clamp recordings, skin-nerve and gut-nerve preparations and in vivo behavioural assessment of pain. Cn2 facilitates Na_V 1.6 early channel opening, and increased persistent and resurgent currents in large-diameter dorsal root ganglion (DRG) neurons. This promotes enhanced excitatory drive and tonic action potential firing in these neurons. In addition, Na_V 1.6 channel activation in the skin and gut leads to increased response to mechanical stimuli. Finally, intra-plantar injection of Cn2 causes mechanical but not thermal allodynia. This study confirms selectivity of Cn2 on Na_V 1.6 channels in sensory neurons. Activation of Na_V 1.6 channels, in terminals of the skin and viscera, leads to profound changes in neuronal responses to mechanical stimuli. In conclusion, sensory neurons expressing Na_V 1.6 are important for the transduction of mechanical information in sensory afferents innervating the skin and viscera.