Malate Dehydrogenases of Ox Kidney

Abstract

Isolation of malate dehydrogenase from ox kidney mitochondria consists of repeated salt and ethanol fractionation and DEAE + CM cellulose chromatography of an extract of mitochondrial acetone powder, resulting in 150‐fold purification and 60% recovery. Molecular and catalytic properties of the isoenzymes of both mitochondrial and cytoplasmic origin are compared in terms of modifiability by phosphate and 1 mM oxalacetate.