Chemical Communications · 2019 · 35 citations · 30 references
EngineeringBiotechnologyMolecular BiologyBioorthogonal ChemistryBiosensorsGene ExpressionDna BiosensorsG-quadruplex ChemistryMicrorna BiologyNanomaterial-based BiosensorsNucleic Acid BiosensorsSignal Amplification StrategiesDna ImmobilizationFluorescent Nucleic Acid BiosensorsFluorescent ProbesImmobilizationMicrorna BiosensorsNucleic Acid AmplificationRolling Circle AmplificationUltrasensitive Detection
In this work, a novel fluorescent assay was proposed for the ultrasensitive detection of microRNA-21 (miRNA-21) based on the efficient immobilization of protoporphyrin IX (PPIX) as signal indicators in massive G-quadruplex structures obtained by target recycling, three-dimensional DNA walker and a rolling circle amplification (RCA) coupled cascade nucleic acid amplification strategy.
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