Seneca Valley virus RNA detection in pig feed and feed ingredients in Brazil

Abstract

We investigated Seneca Valley virus (SVV) contamination in pig feed and feed ingredients. Twenty-seven samples were collected from two Brazilian feed mills and subjected to conventional RT-nested-PCR and qRT-PCR assays. Seven samples were SVV-positive with viral loads of 3.94-4.33 log₁₀ genomic copies/g of feed. The study reveals SVV feed and feed ingredient contamination under natural conditions in Brazil.