Mir-29A Inhibits Invasion and Metastasis of Cervical Cancer Via Modulating Methylation of Tumor Suppressor Socs1

Abstract

<b>Aims:</b> To investigate roles of miR-29a-DNMT1-SOCS1 axis in cervical cancer invasion and migration. <b>Materials & methods:</b> The methylation level of SOCS1 was determined by methylation specific PCR. The cell apoptosis, proliferation, migration and invasion were examined by Annexin-V/PI staining, MTT and colony formation assays, plus scratch and transwell assays respectively. The expressions of epithelial-mesenchymal transition and NF-κB related proteins were determined by western blotting. <b>Results:</b> MiR-29a was downregulated, accompanied with DNMT1 upregulation and SOCS1 downregulation in cervical cancer tissues. MiR-29a suppressed DNMT1, inhibited SOCS1 promoter methylation and upregulated its expression. Moreover, miR-29a promoted cell apoptosis, suppressed proliferation, inhibited migration and invasion via inactivation of NF-κB signaling pathway in cervical cancer cells. <b>Conclusion:</b> MiR-29a-DNMT1-SOCS1 axis plays an important role on invasion and metastasis in cervical cancer via NF-κB signaling pathway.