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PURIFICATION AND SEPARATION OF FOLLICLE STIMULATING HORMONE (FSH) AND LUTEINIZING HORMONE (LH) FROM HUMAN POSTMENOPAUSAL GONADOTROPHIN (HMG)
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1966
Year
FertilityGynecologyReproductive BiologySeparation ScienceBioanalysisAnalytical ChemistrySeparation TechniqueClinical ChemistryPublic HealthPurification MethodAdvanced SeparationPotent Fsh FractionChromatographyEndocrine MechanismLuteinizing HormoneFsh FractionsEndocrinologyUrinary FshUrologyReceptor BiologyMedicineReproductive HormoneGonadotropin Biology
ABSTRACT Results of this study indicate that some methods for purification and separation of urinary FSH and LH are available and that different grades of separation of the two hormones can be achieved depending on the purity of the starting material used. The most potent FSH fraction with the highest FSH/LH ratio was obtained with chromatography on DEAE-C column, followed twice by gel filtration on Sephadex G-100. Nevertheless all the FSH fractions reported are more or less contaminated by luteinizing hormone.