Six New Coumarin Glycosides from the Aerial Parts of Gendarussa vulgaris

Abstract

Six new coumarin glycosides, genglycoside A-F (<b>1</b>-<b>6</b>), were isolated from the aerial parts of <i>Gendarussa vulgaris</i>, along with ten known analogues (<b>7</b>-<b>16</b>). Their structures were unambiguously established on the basis of extensive spectroscopic data and HPLC analysis. The cytotoxic activities of all isolated compounds were evaluated by MTT assay. Compound <b>12</b> showed the most potent cytotoxicity in Eca-109, MCF-7, and HepG2 cell lines. By the preliminary structure-activity relationships, it was firstly discovered that the glycosylation or esterification at 7,8-dihydroxy or 7-hydroxy drastically reduced the cytotoxic activity of the parent coumarin.