Phylogenomics insights into order and families of Lysobacterales

Abstract

Order <i>Lysobacterales</i> (earlier known <i>Xanthomonadales</i> ) is a taxonomically complex group of a large number of gamma-proteobacteria classified in two different families, namely <i>Lysobacteraceae</i> and <i>Rhodanobacteraceae</i> . Current taxonomy is largely based on classical approaches and is devoid of whole-genome information-based analysis. In the present study, we have taken all classified and poorly described species belonging to the order <i>Lysobacterales</i> to perform a phylogenetic analysis based on the 16 S rRNA sequence. Moreover, to obtain robust phylogeny, we have generated whole-genome sequencing data of six type species namely <i>Metallibacterium scheffleri , Panacagrimonas perspica , Thermomonas haemolytica , Fulvimonas soli , Pseudofulvimonas gallinarii</i> and <i>Rhodanobacter lindaniclasticus</i> of the families <i>Lysobacteraceae</i> and <i>Rhodanobacteraceae</i> . Interestingly, whole-genome-based phylogenetic analysis revealed unusual positioning of the type species <i>Pseudofulvimonas</i> , <i>Panacagrimonas , Metallibacterium</i> and <i>Aquimonas</i> at family level. Whole-genome-based phylogeny involving 92 type strains resolved the taxonomic positioning by reshuffling the genus across families <i>Lysobacteraceae</i> and <i>Rhodanobacteraceae</i> . The present study reveals the need and scope for genome-based phylogenetic and comparative studies in order to address relationships of genera and species of order <i>Lysobacterales</i> .