New Journal of Chemistry · 2019 · 28 citations · 32 references
EngineeringBiotechnologyMolecular BiologyBiosensorsMolecular DiagnosticsVirologyAntigen Detection AssaysAptamer-based BiosensorsDna BiosensorsInfectious Disease Molecular TestingNucleic Acid BiosensorsViral DiagnosticsAptamer-based AssaysAptamer-based BiosensingAptamer-based DetectionColorimetric BiosensorsColorimetric SensingDna Aptamer-based BiosensorsNucleic Acid Aptamer SelectionSignal-to-noise Ratio
In this paper, we introduce an effective method for selecting aptamer that increases the signal-to-noise ratio in a heterogenous sandwich-type immunosensor and confirm the efficiency of selected aptamer candidates in the colorimetric assay. Using the proposed method, four aptamer candidates with<italic>K</italic><sub>d</sub>values ranging from 77.6 nM to 125.7 nM were obtained.
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In vitro selection of RNA molecules that bind specific ligands
Andrew D. Ellington, Jack W. Szostak · Nature · 1990 · 9.7K citations