Problems of Cryobiology and Cryomedicine · 2019 · 11 citations · 58 references
This review describes the state of the art in cryopreservation of spermatozoa, oocytes and animal embryos. We have analyzed methodical approaches for the effective low-temperature preservation of the genetic resources of animal world and speciï¬ed the procedures of cryopreservation of animal gametes and embryos. The history of efficient cryopreservation for animal reproductive cells and embryos was described. To date the germ cells and embryos of many laboratory, agricultural and wild animals have been successfully cryopreserved, nevertheless there is still pending the issue with the oocytes and embryos of some species of birds, ï¬ shes and mammals. The paper discusses the ways for developing the methods to preserve the genetic material of those species that have not been successfully cryopreserved yet. Probl Cryobiol Cryomed 2019; 29(1): 003–018
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Survival of mouse embryos frozen to -196 degrees and -269 degrees C.
D. G. Whittingham, S.P. Leibo, P. Mazur · PubMed · 1972 · 782 citations
Survival of Mouse Embryos Frozen to -196° and -269°C
D. G. Whittingham, S.P. Leibo, P. Mazur · Science · 1972 · 736 citations