Real‐Time Mapping of Ultratrace Singlet Oxygen in Rat during Acute and Chronic Inflammations via a Chemiluminescent Nanosensor

Abstract

Sensing nonradiation-induced singlet oxygen (¹ O₂ ) in whole-animal is deemed as one of the most challenging tasks in noninvasive techniques due to the µs level lifetime of ¹ O₂ and quenching by numerous reductants in tissues. Here a distinct chemiluminescent (CL) nanosensor (NTPE-PH) that boasts ultrahigh concentrated CL units in one nanoparticle is reported. Taking advantage of the intramolecular energy transfer mechanism that promises high energy transfer efficiency and the aggregation-induced emission behavior that guarantees high CL amplification, the NTPE-PH sensor is sensitive to a nm level ¹ O₂ . Experiments demonstrate that the NTPE-PH yields a highly selective CL response toward ¹ O₂ among common reactive oxygen species. With proved low cytotoxicity and good animal compatibility, real-time mapping of ultratrace ¹ O₂ in whole-animal during acute and chronic inflammations is first achieved. It is anticipated that the NTPE-PH sensor can be a useful tool for monitoring ¹ O₂ variation during immune response and pathological processes corresponding to different stimuli, even with drug treatment included.