Enhanced Pb immobilization via the combination of biochar and phosphate solubilizing bacteria

Abstract

Application of biochar in heavy metal remediation suffers from lack of long-term stability. Phosphate-solubilizing bacteria (PSB) are able to elevate P release and the subsequent reaction with Pb to form stable pyromorphite. This study investigated the feasibility of applying PSB modified biochar to enhance immobilization of Pb²⁺. An alkaline biochar produced from rice husk (RB) and a slightly acidic biochar produced from sludge (SB) were selected. It showed that the biochars can effectively remove Pb²⁺ via adsorption, i.e., aqueous Pb concentrations after RB and SB addition were reduced by 18.61 and 53.89% respectively. The addition of PSB increased the Pb²⁺ removal for both biochars (to 24.11 and 60.85%, respectively). In particular, PSB significantly enhanced the formation of stable pyromorphite on surface of SB. This is due to that the evenly distributed PSB enhanced P release and regulated pH on the biochar surface. Moreover, small particles (<0.074 mm) showed their higher ability to induce the formation of pyromorphite, for both RB and SB. Nevertheless, SB demonstrated higher capability of sorption, together with its more abundant P content, which provided a more suitable platform to attract PSB to immobilize heavy metals. Therefore, the combination of biochar and PSB is a promising candidate material for heavy metal remediation. However, the types and particle size distribution of biochar should be addressed.