A new sesquiterpenoid with cytotoxic and anti-inflammatory activity from the leaves of <i>Datura metel</i> L

Abstract

A new sesquiterpenoid glycoside, consisting of one vetispirane-type, dmetelisproside A (<b>1</b>), together with two known megastigmane sesquiterpenoids, citroside A (<b>2</b>) and staphylionoside D (<b>3</b>) were isolated from the leaves of <i>Datura metel</i> L. The structures were determined by analysis of their MS and NMR data as well as by comparison with literature values. All isolates were evaluated for cytotoxicity toward SGC-7901, Hepg2, HeLa, MCF-7, and MDA-MB-231 cancer cell lines, and anti-inflammatory activity against nitric oxide (NO) in RAW 264.7 cells <i>in vitro</i>. Compounds <b>1</b> and <b>2</b> exhibited pronounced cytotoxicity against SGC-7901 and HeLa cells with IC₅₀ values in the range of 21.43 to 29.51 μm. All three compounds displayed potential effects against NO production with IC₅₀ values of 31.10, 34.25, and 44.31 μm, respectively. Thus, these isolated compounds in the leaves of <i>Datura metel</i> L were subjected to bioactivity substances for future studies on medicinal application.