Multiple amino acid substitutions involved in the adaption of three avian-origin H7N9 influenza viruses in mice

Abstract

Our result indicated that the virulence in mice and virus titer in MDCK cells of H7N9 viruses significantly increased after adapted in mouse model. PB2 E627K and PA T97A substitutions are vital in mouse adaption and should be monitored during epidemiological study of H7N9 virus.