bioRxiv (Cold Spring Harbor Laboratory) · 2019 · 21 citations · 36 references
Life SciencesBiologyZoologyGene ExpressionGenomicsTranscriptomicsAnimal GenomicsMolluscan BiologyVenom BiologyIon Channel SelectivityMolluscan GenomicsReceptor SuperfamiliesSelectivitySnake Venom ProteomicsTranscriptome SequencingVenom Gene ExpressionVenom Transcriptomics
Abstract Background Conus consors is a fish-hunting cone snail that lives in the tropical waters of the Indo-Pacific region. Cone snails have attracted scientific interest for the amazing potency of their venom, which consists of a complex mixture of small proteins known as conopeptides, many of which act as ion channel and receptor modulators with high selectivity. Results We have analysed publicly available transcriptomic sequences from 8 tissues of Conus consors and complemented the transcriptome data with the data from genomic DNA reads. We identified 17,715 full-length protein sequences from the transcriptome. In addition, we predicted 168 full-length or partial conopeptide sequences and characterized gene structures of several conopeptide superfamilies.
36
Full-length transcriptome assembly from RNA-Seq data without a reference genome
Manfred Grabherr, Brian J. Haas, Moran Yassour et al. · Nature Biotechnology · 2011 · 22.6K citations · Full text
Accelerated Profile HMM Searches
Sean R. Eddy · PLoS Computational Biology · 2011 · 7.5K citations · Full text