<p>Characterization of a novel <em>bla</em><sub>NDM-5</sub>-harboring IncFII plasmid and an <em>mcr-1</em>-bearing IncI2 plasmid in a single <em>Escherichia coli</em> ST167 clinical isolate</p>

Abstract

Background and purpose: The spread of the plasmid-mediated, colistin-resistance gene mcr-1 into New Delhi metallo-β-lactamase (NDM)-producing bacterial isolates can cause untreatable infections. In this study, we conducted a molecular characterization of a novel, conjugative, bla NDM-5 -positive IncFII plasmid (pNDM-EC16-50) together with an mcr-1 -bearing IncI2 plasmid in a single Escherichia coli ST167 clinical isolate EC16-50. Methods and results: EC16-50, which belongs to the E. coli strain ST167 and phylogroup A, was identified to co-produce NDM-5 and MCR-1. S1-PFGE and Southern blotting showed that bla NDM-5 and mcr-1 genes were located on ~95 kb and ~65 kb plasmids, respectively. A conjugation assay revealed that both bla NDM-5 - and mcr-1 -bearing plasmids were self-transmissible. Comparative plasmid analysis suggested that bla NDM-5 -harboring F2:A-:B-plasmid might have evolved from the well-reported NDM-carrying pMC-NDM-like plasmid via recombination with a locally emerged pSJ_94 plasmid, whereas the mcr-1 -carrying IncI2 plasmid was similar to previously reported mcr-1 -bearing plasmids in China. Conclusion and impact: This study represents the first report of the NDM-5 carrying IncFII- but not IncX3-type plasmid in an MCR-1-producing E. coli isolate. More striking was the dissemination of mcr-1 in a successful epidemic NDM-5-producing E. coli clone ST167, which could facilitate the spread of colistin resistance in carbapenemase-producing E. coli isolates. Keywords: Escherichia coli , ST167, MCR-1, IncFII plasmid, NDM-5