Adipose‐derived stem cells attenuate atopic dermatitis‐like skin lesions in <scp>NC</scp>/Nga mice

Abstract

Abstract There is an unmet need in novel therapeutics for atopic dermatitis ( AD ). We examined the effects of autologous adipose‐derived stem cells ( ADSC s) on AD ‐like skin lesions induced by the application of 2,4‐dinitrochlorobenzene ( DNCB ) in NC /Nga mice. Autologous ADSC s and ADSC ‐conditioned medium ( ADSC ‐ CM ) were injected intralesionally three times. Clinical severity and histopathologic findings were compared in sham naïve control, saline‐treated, ADSC ‐treated, ADSC ‐ CM ‐treated and 2.5% cortisone lotion‐applied animals. The severity index, skin thickness, mast cell number, as well as expression levels of thymic stromal lymphopoietin, CD 45, chemoattractant receptor‐homologous molecule, chemokine ligand 9 and chemokine ligand 20 were significantly lower in mice treated with ADSC , ADSC ‐ CM , or 2.5% cortisone lotion. Tissue levels of interferon‐γ as well as serum levels of interleukin‐33 and immunoglobulin E levels were also decreased in those groups. We conclude that autologous ADSC s improved DNCB ‐induced AD ‐like skin lesions in NC /Nga mice by reducing inflammation associated with Th2 immune response and interferon‐γ.