Journal of Cancer · 2019 · 38 citations · 41 references
MedicineMolecular BiologyOncologyGene ExpressionMolecular OncologyTranscriptional RegulationCancer Cell SignalingMyc Family OncogenesNoncoding Rna FunctionNoncoding Rna In CancerOncogene ActivationOncogene OverexpressionCancer BioinformaticsLong Noncoding Rna RegulationMinimal Promoter ArchitectureMyc In Tumor InitiationOncogenic Myc SignalingPromoter Deletion AnalysisPromoter Region IdentificationTherapeutic Gene Expression
Actin filament-associated protein 1-antisense RNA1 (AFAP1-AS1), a cancer-related long non-coding RNA, has been found to be upregulated in multiple types of cancers. AFAP1-AS1 is important for the initiation, progression and poor prognosis of many cancers, including nasopharyngeal carcinoma (NPC). However, the mechanism underlying the regulation of AFAP1-AS1 expression is not well-understood. In our study, the potential promoter region of AFAP1-AS1 was predicted by comprehensive bioinformatics analysis. Moreover, promoter deletion analysis identified the sequence between positions -359 and -28 bp as the minimal promoter region of AFAP1-AS1. The ChIP assay results indicate that the AFAP1-AS1 promoter is responsive to the transcription factor c-Myc, which can promote high AFAP1-AS1 expression. This study is the first to clone and characterize the AFAP1-AS1 promoter region. Our findings will help to better understand the underlying mechanism of high AFAP1-AS1 expression in tumorigenesis and to develop new strategies for therapeutic high expression of AFAP1-AS1 in NPC.
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TP53 loss creates therapeutic vulnerability in colorectal cancer
Yunhua Liu, Xinna Zhang, Cecil Han et al. · Nature · 2015 · 245 citations