Photorhabdus khanii subsp. guanajuatensis subsp. nov., isolated from Heterorhabditis atacamensis, and Photorhabdus luminescens subsp. mexicana subsp. nov., isolated from Heterorhabditis mexicana entomopathogenic nematodes

Abstract

Two Gram-negative, rod-shaped, non-spore-forming bacteria, MEX20-17^T and MEX47-22^T, were isolated from the digestive system of Heterorhabditis atacamensis and Heterorhabditis mexicana entomopathogenic nematodes, respectively. Their 16S rRNA gene sequences suggest that strains MEX20-17^T and MEX47-22^T belong to the γ-Proteobacteria and to the genus Photorhabdus. Deeper analyses using housekeeping-gene-based and whole-genome-based phylogenetic reconstruction suggest that MEX20-17^T is closely related to Photorhabdus khanii and that MEX47-22^T is closely related to Photorhabdus luminescens. Sequence similarity scores confirm these observations: MEX20-17^T and P. khanii DSM 3369^T share 98.9 % nucleotide sequence identity (NSI) of concatenated housekeeping genes, 70.4 % in silico DNA-DNA hybridization (isDDH) and 97 % orthologous average nucleotide identity (orthoANI); and MEX47-22^T and P. luminescens ATCC 29999^T share 98.9 % NSI, 70.6 % isDDH and 97 % orthoANI. Physiological characterization indicates that both strains differ from all validly described Photorhabdus species and from their more closely related taxa. We therefore propose to classify MEX20-17^T and MEXT47-22^T as new subspecies within P. khanii and P. luminescens, respectively. Hence, the following names are proposed for these strains: Photorhabdus khanii subsp. guanajuatensis subsp. nov. with the type strain MEX20-17^T (=LMG 30372^T=CCOS 1191^T) and Photorhabdus luminescenssubsp. mexicana subsp. nov. with the type strain MEX47-22^T (=LMG 30528^T=CCOS 1199^T). These propositions automatically create Photorhabdus khanii subsp. khanii subsp. nov. with DSM 3369^T as the type strain (currently classified as P. khanii), and Photorhabdus luminescenssubsp. luminescenssubsp. nov. with ATCC 29999^T as the type strain (currently classified as P. luminescens).