Helios ⁺ and Helios ⁻ Treg subpopulations are phenotypically and functionally distinct and express dissimilar TCR repertoires

Abstract

Abstract The transcription factor Helios is expressed in a large subset of Foxp3 + Tregs. We previously proposed that Helios is a marker of thymic derived Treg (tTreg), while Helios − Treg were induced from Foxp3 − T conventional (Tconv) cells in the periphery (pTreg). To compare the two Treg subpopulations, we generated Helios‐GFP reporter mice and crossed them to Foxp3‐RFP reporter mice. The Helios + Treg population expressed a more activated phenotype, had a slightly higher suppressive capacity in vitro and expressed a more highly demethylated TSDR but were equivalent in their ability to suppress inflammatory bowel disease in vivo. However, Helios + Treg more effectively inhibited the proliferation of activated, autoreactive splenocytes from scurfy mice. When Helios + and Helios − Treg were transferred to lymphoreplete mice, both populations maintained comparable Foxp3 expression, but Foxp3 expression was less stable in Helios − Treg when transferred to lymphopenic mice. Gene expression profiling demonstrated a large number of differentially expressed genes and showed that Helios − Treg expressed certain genes normally expressed in CD4 + Foxp3 − T cells. TCR repertoire analysis indicated very little overlap between Helios + and Helios − Treg. Thus, Helios + and Helios − Treg subpopulations are phenotypically and functionally distinct and express dissimilar TCR repertoires.