Mutations in pheromone‐binding protein3 contribute to pheromone response variations in <i>Plutella xylostella</i> (L.) (Lepidoptera: Plutellidae)

Abstract

Abstract BACKGROUND Diamondback moth Plutella xylostella (L.) (Lepidoptera: Plutellidae) is one of the most important crucifer pests. Commercial sex attractants have been developed to monitor and control P. xylostella . However, some studies have demonstrated a variety of pheromone responses of P. xylostella in different locations of the world. Soluble pheromone‐binding proteins (PBPs), as a subfamily of odorant‐binding proteins (OBPs), could selectively bind and transport pheromones across aqueous sensillar lymph to the surface of olfactory receptor neurons. It is worthy to study whether the mutation of Pxyl PBPs is one of the reasons for the different responses of sex attractors in different regions. RESULTS In this study, P. xylostella males were collected from seven Chinese provinces, including Hainan, Guangdong, Yunnan, Fujian, Hunan, Zhejiang, and Hebei. Pxyl PBP1, Pxyl PBP2, and Pxyl PBP3 were cloned, and 3, 6, and 32 types of mutation pattern were identified, respectively. These mutation patterns were distributed in each province with different frequency. The results of fluorescence displacement binding assay and in silico simulation revealed that the three mutant Pxyl PBP3 were more sensitive to Z11‐16:Ald than the reference protein (ACI28451). CONCLUSION This result implied that mutation of Pxyl PBP3 may have contributed to regional differences in pheromone responses of P. xylostella . © 2019 Society of Chemical Industry