Identification and cloning of heat shock protein 70 gene from scallop Chlamys farreri

Abstract

Expressed sequence tagging method was used to identify the partial sequences of HSP70 from Chlamys fareri, then the full length cDNA was cloned through the approach of Anchored-PCR. The full length of HSP70 cDNA from Chlamsy farreri was 2553bp and encoded 634 amino acids. BLAST analysis revealed that the HSP70 gene from C. farreri shared high identity with the HSP70 genes from other organisms in nucleotides and amino acids respectively. Three signature sequences belonging to HSP70 family were detected in the cDNA sequence from Chlamy farreri. The result indicated that the cDNA sequence cloned from C. farreri was a member of heat shock protein 70 family, it could be used in the study of the anti-stress mechanism in scallop, and the genetic breeding or improvement.