A new ocotillol-type ginsenoside from stems and leaves of <i>Panax quinquefolium</i> L. and its anti-oxidative effect on hydrogen peroxide exposed A549 cells

Abstract

A new ocotillol-type ginsenoside, namely 12-one-pseudoginsenoside F₁₁ (12-one-PF₁₁), was isolated from stems and leaves of <i>Panax quinquefolium</i>, whose structure was elucidated 6-<i>O</i>-[<i>α</i>-L-rhamnopyranosyl-(1-2)-<i>β</i>-D-glucopyranosyl]-dammar-12-one-20S,24R-epoxy-3<i>β</i>,6<i>α</i>,25-triol. 12-one-PF₁₁ significantly suppressed hydrogen peroxide induced oxidative stress in human lung carcinoma A549 cells. As compared with model group, 12-one-PF₁₁ improved cell viability of A549 cells in a dose-dependent manner, and significantly decreased the generation of malondialdehyde (MDA) and increased production of superoxide dismutase (SOD) and glutathione (GSH) and protein expression levels of nuclear related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) in A549 cells.