Characterization of a Microalgal UV Mutant for CO₂ Biofixation and Biomass Production

Abstract

The mutagenesis is an emerging strategy for screening microalgal candidates for CO 2 biofixation and biomass production. In this study, by 96-well microplates-UV mutagenesis, a mutant stemmed from Scenedesmus obliquus was screened and named as SDEC-1M. To characterize SDEC-1M, it was cultivated under air and high level CO 2 (15% v/v), and its parental strain (PS) was considered as control. Growth characterizations showed that SDEC-1M grew best in high level CO 2 . It indicated that the mutant had high CO 2 tolerance (HCT) and growth potential under high level CO 2 . Richer total carbohydrate content (37.26%) and lipid content (24.80%) demonstrated that, compared to its parental strain, SDEC-1M was apt to synthesize energy storage materials, especially under high CO 2 level. Meanwhile, the highest light conversion efficiency (approximately 18 %) was also obtained. Thus, the highest overall biomass productivities were achieved in SDEC-1M under high level CO 2 , largely attributed to that the highest productivities of total lipid, total carbohydrate, and crude protein were also achieved in the meantime. By modified UV, therefore, mutagenized SDEC-1M was the better candidate for CO 2 biofixation and biofuel production than its parental strain.