A new sesquiterpene, a new monoterpene and other constituents with anti-inflammatory activities from the roots of <i>Aristolochia debilis</i>

Abstract

A new sesquiterpene (<b>1</b>) and a new monoterpene (<b>2</b>), together with thirteen known compounds (<b>3</b>-<b>15</b>) were isolated from an ethanol extract of the roots of <i>Aristolochia debilis</i> Sieb. et Zucc. The structures of compounds <b>1</b> and <b>2</b> were elucidated using HR-ESI-MS, 1D and 2D NMR spectroscopy. Anti-inflammatory effects of the isolated compounds were evaluated in terms of inhibition of nitric oxide, tumour-necrosis factor-α and interleukin-6 production in lipopolysaccharide-stimulated RAW264.7 cells. Compounds <b>1</b>-<b>9</b> and <b>12</b>-<b>15</b> significantly inhibited the levels of NO, TNF-α and IL-6 in LPS-induced RAW264.7 cells from concentrations of 3 μM to 30 μM.