Phytochemical screening and antimicrobial activity of wild and tissue cultured plant extracts of Tylophora indica

Abstract

Objective: To analyze phytochemical of wild and tissue cultured plant ethanol and methanol extracts of Tylophora indica through FTIR, GCMS and evaluation of antimicrobial activity against four human pathogens. Materials and Methods: Preliminary phytochemical screening was done by standard methods. Analysis of the wild and tissue culture plants were carried out through the potassium bromide (KBr) pellet (FTIR grade) method. The GC -MS analysis was carried out using a Clarus 500 Perkin -Elmer (Auto system XL) Gas Chromatograph equipped and coupled to a mass detector. Antimicrobial activity of ethanol and methanol extracts of wild and tissue cultured plant samples in Tylophora indica against bacterial pathogens and also two fungal pathogens by agar well diffusion method.