Evaluation of the effect of BMSCs condition media and methylprednisolone in TGF-β expression and functional recovery after an acute spinal cord injury

Abstract

The purpose of this experimental study is evaluation of the effect of BMSCs Condition Media and Methylprednisolone in TGF-β expression and functional recovery after acute spinal cord injury in adult wistar rat. MATERIAL AND METHODS: After an acute spinal cord injury, MP and BMSC-CM were injected intraperitoneally and the recovery rate was evaluated by BBB and narrow beam test. Real time PCR, H[et]E staining, cavity formation and stereology was done after 12 weeks post injury. RESULT: There were significant differences in functional recovery between MP+CM group, relative to other groups. TGF-β1 expression decreased in MP+CM group compared to the control group. Cavity size in experimental groups decreased compared to the control group. The mean volume of spinal cord demonstrated a significant increase in the MP+CM group in comparison to the other experimental groups. CONCLUSION: Simultaneous use of MP and BMSC-CM will improve recovery from the spinal cord injury, reduce inflammation and improve functional recovery (Tab. 1, Fig. 8, Ref. 26).