Evaluation of Antibacterial Activity and Drug Release Behavior of Chitosan-Based Nanofibers (In Vitro Study)

Abstract

Spinning of chitosan-poly (vinyl alcohol); Cs/PVA and chitosan- poly (caprolactone); Cs/PCL polymer blends were successfully achieved using electrospinning technique. The nanofiber mats of Cs/PVA and Cs/PCL were used as carriers for examining the release profile of Ciprofloxacin.HCl as broad-spectrum antibiotic. The shape, size, structure and composition of the nanofibers were characterized using FT-IR Spectroscopy and Scanning Electron Microscope (SEM). The release behavior was studied in Phosphate Buffer Solution (pH=7.4) using a UV-spectrophotometer. The antimicrobial activity of the produced nanofiber mats was studied using different strains of human pathogenic bacteria and Fungi. Compared to release profile from Cs/PVA/Ciprofloxacin.HCl nanofibers show faster drug release than that of Cs/PCL/Ciprofloxacin.HCl system,indicating that these nanofiber mats are promising active wound dressing materials for wound dressing applications.