The antioxidant rich active principles of Clerodendrum sp. controls haloalkane xenobiotic induced hepatic damage in murine model

Abstract

<i>Clerodendrum</i> is a plant with potent antioxidant activity and has been frequently employed as a traditional remedy against bronchitis, asthma, liver and stomach disorders. Three species of genus <i>Clerodendrum</i> namely <i>Clerodendrum indicum, C. colebrookianum</i> and <i>C. inerme</i> (Syn. <i>Volkameria inermis</i>) were investigated for their possible activity against oxidative stress induced liver injury. Apart from generation of Reactive Oxygen Species (ROS) in the WRL-68 cell line (human hepatic cell line), <i>in-vitro</i> and <i>in-vivo</i> antioxidant assays were also assessed. Features of immune cell proliferation (MTT) were analyzed thoroughly. Gas Chromatography-Mass Spectrometry (GC-MS) and Fourier Transform Infrared Spectroscopy (FTIR) analyses have been performed to identify the active biological compounds. These active biological compounds were further subjected to molecular docking. The antioxidant activity of three <i>Clerodendrum</i> sp. was significantly high in DPPH, nitric oxide, hydroxyl radical and hydrogen peroxide etc. Biochemical parameters like catalase, superoxide dismutase (SOD) and reduced glutathione (GSH) were generated in excess due to CCl₄ administration, which was ameliorated by treating with <i>Clerodendrum</i> extract. The phytochemical 24,25-Dihydroxyvitamin D shows excellent binding affinity in Autodock Vina. The present study provided convincing evidences that <i>C. indicum</i> and <i>C. inerme</i> showed good result but <i>C. colebrookianum</i> performed better by almost all means.