Acylphloroglucinol Derivatives from Garcinia multiflora with Anti-Inflammatory Effect in LPS-Induced RAW264.7 Macrophages

Abstract

Two new acylphloroglucinol derivatives, 13,14-didehydroxygarcicowin C (<b>1</b>) and 13,14-didehydroxyisoxanthochymol (<b>2</b>), have been isolated from the stems of <i>Garcinia multiflora</i>, together with seven known compounds (<b>3</b>⁻<b>9</b>). The structures of new compounds <b>1</b> and <b>2</b> were elucidated by MS and extensive 1D/2D NMR spectroscopic analyses. Among the isolates, 13,14-didehydroxy-isoxanthochymol (<b>2</b>) and sampsonione B (<b>3</b>) exhibited inhibition against lipopolysaccharide (LPS)-induced NF-κB activation in macrophages at 30 μM with relative luciferase activity values (inhibitory %) of 0.75 ± 0.03 (24 ± 4%) and 0.12 ± 0.03 (88 ± 4%), respectively. Additionally, sampsonione B (<b>3</b>) reduced LPS-induced nitric oxide (NO) production in murine RAW264.7 macrophages and did not induce cytotoxicity against RAW 264.7 cells after 24 h treatment. Compound <b>3</b> is worth further investigation and may be expectantly developed as an anti-inflammatory <i>drug candidate.</i>