Genomic Characterization of Carbapenemase-Producing Klebsiella pneumoniae with Chromosomally Carried <i>bla</i> _NDM-1

Abstract

We report here <i>Klebsiella pneumoniae</i> strains carrying chromosomal <i>bla</i>_NDM-1 in Thailand. The genomes of these two isolates include a 160-kbp insertion containing <i>bla</i>_NDM-1, which is almost identical to that in the IncHI1B-like plasmid. Further analysis indicated that IS<i>5</i>-mediated intermolecular transposition and Tn<i>3</i> transposase-mediated homologous recombination resulted in the integration of <i>bla</i>_NDM-1 into the chromosome from an IncHI1B-like plasmid. The spread of this type of carbapenem-resistant <i>Enterobacteriaceae</i> may threaten public health and warrants further monitoring.