Thermal expansion of substrate may affect adhesion of Chinese hamster fibroblasts to surfaces during freezing

Abstract

/K). V79-4 Chinese hamster fibroblasts were cultured on both glass and Rinzl coverslips until confluent and the area of coverage was measured before and after freezing at -9 °C. The glass coverslips showed significant loss of cells (coverage = 77.9 ± 8.0%) compared with Rinzl (coverage = 97.9 ± 1.4%). We concluded that Rinzl coverslips may improve cell attachment in future monolayer cryopreservation experiments.