Superresolution Diffuse Optical Imaging by Localization of Fluorescence

Abstract

Diffuse optical imaging has strong potential for $i\phantom{\rule{0}{0ex}}n$ $v\phantom{\rule{0}{0ex}}i\phantom{\rule{0}{0ex}}v\phantom{\rule{0}{0ex}}o$ and deep-tissue applications in medicine, but has been hampered by low spatial resolution. The authors present a superresolution method for diffuse optical imaging that is based on point localization in a diffusion framework, and they demonstrate an improvement in resolution of over two orders of magnitude when imaging fluorescence. This approach will enable optical imaging through centimeters of tissue, at a resolution of tens of microns.