Detection of Volatile Constituents from Food Lures by Tephritid Fruit Flies

Abstract

Tephritid fruit flies require protein for sexual and gonotrophic development. Food-based lures are therefore widely used in strategies to detect and control fruit flies in the Tephritidae family. However, these baits are attractive to a broad range of insect species. We therefore sought to identify volatiles detected by the fly antennae, with the goal to compose lures that more specifically target tephritids. Using gas chromatography-coupled electroantennographic detection (GC-EAD) we screened for antennal responses of four important tephritid species to volatile compounds from five commercially available protein-based baits. Antennal active compounds were reconstituted in synthetic blends for each species and used in behavioral assays. These species-based blends were attractive in olfactometer experiments, as was a blend composed of all antennally active compounds from all the four species we observed (tested only in <i>Bactrocera dorsalis</i>, Hendel). Pilot field tests indicate that the blends need to be further evaluated and optimized under field conditions.