Molecular Marker Assisted Confirmation of Hybridity in Indian mustard (Brassica juncea L.)

Abstract

The present study was undertaken to confirm the hybridity of Brassica juncea L. F1 plants based on the amplification pattern of SSR markers. The complementary banding pattern of the male and female parents helped to confirm the genuineness of F1 plants developed. The seeds of two genotypes of Brassica juncea, RSPR-01 used as female parent and Donskaja-IV used as male parent were raised in pots under field conditions and crossed to develop F1 seeds. Out of 20 random SSR primers used for the screening of parental genotypes for polymorphism, 5 primers were found polymorphic. Based on the complementary banding patterns between the hybrid plants and parents, the polymorphic SSR markers BR_A04_9627743 and BR_A01_13393871 were identified as the specific markers which enable to distinguish and identify hybrid form their parental lines. The expression of both the parental alleles in six out of the seven plants confirmed their true hybrid nature.