Development of sensitive dd<scp>PCR</scp> assays to reliably quantify the proviral <scp>DNA</scp> reservoir in all common circulating <scp>HIV</scp> subtypes and recombinant forms

Abstract

The results demonstrate the need for a careful validation of proviral reservoir quantification assays prior to investigations into non-B subtype reservoirs. The LTR and JO assays can sensitively and reliably quantify HIV DNA in a panel that represents the worldwide most prevalent subtypes and CRFs (A, B, C, D, AE, F, G and AG), justifying their application in future trials aimed at global HIV cure.