Involvement of Calcium and Calmodulin in Nitric Oxide-Regulated Senescence of Cut Lily Flowers

Abstract

Both nitric oxide (NO) and calcium ion (Ca²⁺)/calmodulin (CaM) have been shown to regulate the senescence of cut flowers. However, not much is known about the crosstalk between NO and Ca²⁺/CaM during the senescence of cut flowers. In this study, cut Oriental × Trumpet hybrid lily "Manissa" were used to investigate the roles and relationship between NO and Ca²⁺/CaM during postharvest freshness. The results show that the effects of CaCl₂ or NO donor SNAP on the vase life, maximum flower diameter and hours until full opening were dose-dependent, with an optimum concentration of 20 mM CaCl₂ or 100 μM SNAP. However, Ca²⁺ chelators EGTA or BAPTA/AM, Ca²⁺ channel inhibitors LaCl₃ or nifedipine and CaM antagonists W-7 or TFP inhibited the promotion of SNAP. SNAP applied alone significantly increased the endogenous Ca²⁺/CaM contents in cut lily flowers, while EGTA, BAPTA/AM, LaCl₃, nifedipine, W-7, and TFP decreased the advancement of SNAP. In addition, the SNAP-induced Ca²⁺-ATPase activity was more than twice as much as the control, but EGTA, BAPTA/AM, LaCl₃, nifedipine, W-7, and TFP also reversed the enhancement. Moreover, EGTA, BAPTA/AM, LaCl₃, nifedipine, W-7, and TFP prevented the SNAP-induced upregulation of gene expression of <i>CaM</i>, <i>CBL1</i>, and <i>CBL3</i>, which is associated with calcium signaling pathway. Overall, these results suggest that Ca²⁺/CaM may function as downstream molecules in NO-regulated senescence of cut flowers.