Contamination in a Microbiological Laboratory

Abstract

Contamination is undesired introduction of impurities like chemical, microbial or physical matter, into or onto a starting or intermediating cell culture during sampling, holding, processing, storing, transferring, packaging and transporting. A cell culture contaminant is defined as some element in the culture system that is undesirable because of its possible adverse effects on either the system or its use. Contamination of cell cultures is easily the most common problem encountered in several microbial laboratories, sometimes with very serious consequences. In overall, the reason for contaminants are mostly happened through avoidable procedural errors and misguide techniques. Microbes are widely distributed in the environment without restriction and they are involved in different places even inside a laboratory. Microbial contamination is one of a biggest worldwide obstacle for researchers working with microbial cultures. It may lose laboratory valuable strains. False -positive cultures are reports of microbiological laboratory came from due to common and unusual laboratory contaminants. High microbial contaminate concentration is happened in a microbial lab due to lack of proper management. It is a global concern regarding health and leads to difficult for getting accurate research output. It is manually or systematically introduce in our culture and damage the quality of our work. Recently, many articles are mentioned it and previous reports act as evidence for the challenge. In the end, in order to reduce their first apply good laboratory practice next follow appropriate instructions [2, 6, 7 and 12]. Contaminates are receiving high attention, but the sources and processes of them are not well understood. The major methodological part of the study on the basis of an observational cohort study and long year visual investigation experience in the work area (laboratory). The aim of the current study was identified the critical sources of bacterial contamination inside laboratory then it forward answers for causes of cross-contamination how it happens? The purpose of this paper to describe appropriate and necessary options to prevent microbial, physical or chemical cross-contamination and to reduce false positive culture reports as well as to maximize the true result comes from the microbiological laboratory through practical techniques.